Document Type : Research articles
Authors
1 Department of Biology, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran
2 Department of Biology, Izeh Branch, Islamic Azad University, Izeh, Iran
Abstract
Background: Based on traditional beliefs, Biarum carduchrum (family Araceae) can strengthen the nervous system and prevent from sleep disorder and agitation.
Objectives: This study was performed to investigate the protective effects of the ethyl acetate extract of Biarum carduchrum leaves against pentylenetetrazol (PTZ)-induced seizure in rats.
Methods: The present experimental research was conducted on 50 male Wistar rats in Iran during 2019. The animals were examined in five groups of control, PTZ, intervention (PTZ with 100 and 200 mg/kg ethyl acetate extract for 10 days), diazepam (PTZ, 200 mg/kg extract, and diazepam). The research groups were compared in terms of behaviors. Furthermore, total phenol and flavonoid levels in the extract were determined using high-performance liquid chromatography.
Results: According to the results, the intervention and diazepam groups had a significantly lower number of the whole body, tonic, head, and upper organ seizures (P<0.05), and jumps (P<0.05), compared to the PTZ group. Both doses of the extract also significantly decreased immobility time in tail suspension and secondary latency time and significantly enhanced spatial memory in Morris water maze test (P<0.05). This treatment also significantly reduced the levels of nitric oxide and malondialdehyde in the brain and serum (P<0.05) and showed antioxidant activity (IC50=200 ?g/ml). The extract contained 42.63±0.7494 and 85.16±6.499 µg/mg phenol and flavonoid, respectively. The HPLC analysis also revealed the presence of quercetin (30 ?g/g) in the extract.
Conclusions: Based on the results, Biarum carduchrum extract can be used for depression control and improvement of learning and memory impairments in seizure patients after complementary testing.
Keywords
- Devi PU, Manocha A, Vohora D. Seizures, antiepileptics, antioxidants and oxidative stress: an insight for researchers. Expert Opin Pharmacother. 2008;9(18):3169-77. doi: 10.1517/14656560802568230. [PubMed: 19040338].
- Cendes F, Andermann F, Gloor P, Evans A, Jones-Gotman M, Watson C. MRI volumetric measurement of amygdala and hippocampus in temporal lobe epilepsy. Neurology. 1993;43(4):719-25. doi: 10.1212/wnl.43.4.719. [PubMed: 8469329].
- Schouten A, Oostrom K, Pestman W, Peters A, Jennekens-Schinkel A. Learning and memory of school children with epilepsy: a prospective controlled longitudinal study. Dev Med Child Neurol. 2002;44(12):803-11. doi: 10.1017/s0012162201002973. [PubMed: 12455856].
- Bashkatova V, Narkevich V, Vitskova G, Vanin A. The influence of anticonvulsant and antioxidant drugs on nitric oxide level and lipid peroxidation in the rat brain during penthylenetetrazole-induced epileptic form model seizures. Prog Neuro Psychopharmacol Biol Psych. 2003;27(3):487-92. doi: 10.1016/S0278-5846(03)00037-X.
- Gupta Y, Kumar MV, Srivastava A. Effect of Centella asiatica on pentylenetetrazole-induced kindling, cognition and oxidative stress in rats. Pharmacol Biochem Behav. 2003;74(3):579-85. doi: 10.1016/s0091-3057(02)01044-4. [PubMed: 12543222].
- Patsoukis N, Zervoudakis G, Georgiou CD, Angelatou F, Matsokis NA, Panagopoulos NT. Effect of pentylenetetrazol-induced epileptic seizure on thiol redox state in the mouse cerebral cortex. Epilepsy Res. 2004;62(1):65-74. doi: 10.1016/j.eplepsyres.2004.08.005. [PubMed: 15519133].
- Helmstaedter C. Effects of chronic epilepsy on declarative memory systems. Prog Brain Res. 2002;135:439-53. doi: 10.1016/S0079-6123(02)35041-6. [PubMed: 12143363].
- Feng Y, LeBlanc MH, Regunathan S. Agmatine reduces extracellular glutamate during pentylenetetrazole-induced seizures in rat brain: a potential mechanism for the anticonvulsive effects. Neurosci Lett. 2005;390(3):129-33. doi: 10.1016/j.neulet.2005.08.008. [PubMed: 16125317].
- Li ZP, Zhang XY, Lu X, Zhong MK, Ji YH. Dynamic release of amino acid transmitters induced by valproate in PTZ-kindled epileptic rat hippocampus. Neurochem Int. 2004;44(4):263-70. doi: 10.1016/s0197-0186(03)00148-7. [PubMed: 14602089].
- Stamler JS, Simon DI, Jaraki O, Osborne JA, Francis S, Mullins M. S-nitrosylation of tissue-type plasminogen activator confers vasodilatory and antiplatelet properties on the enzyme. Proc Natl Acad Sci U S A. 1992;89(17):8087-91. doi: 10.1073/pnas.89.17.8087. [PubMed: 1325644].
- Wasterlain CG, Fujikawa DG, Penix L, Sankar R. Pathophysiological mechanisms of brain damage from status epilepticus. Epilepsia. 1993;34(Suppl 1):S37-53. doi: 10.1111/j.1528-1157.1993.tb05905.x. [PubMed: 8385002].
- Liang LP, Patel M. Seizure-induced changes in mitochondrial redox status. Free Radic Biol Med. 2006;40(2):316-22. doi: 10.1016/j.freeradbiomed.2005.08.026. [PubMed: 16413413].
- Boyce PCA. Taxonomic revision of Biarum: Araceae. Curtis Bot Mag. 2000;25(1):2-17. doi: 10.1111/j.1467-8748.2007.00607.x.
- Hegnauer R. Phytochemistry and chemotaxonomy of the Araceae. Aroideana. 1987;10(2):17-9.
- Hosseini E, Rousta E, Loghmany FT, Mahmoudpour M. In vitro antioxidant activity of hydromethanolic extract of Karde (Biarumcarduchrum) and Tts effects on the serum lipids of rats. Iran J Nutr Sci Food Technol. 2014;9(3):1-8.
- Rabiei Z, Rafieian-Kopaei M, Heidarian E, Saghaei E, Mokhtari S. Effects of Zizyphus jujube extract on memory and learning impairment induced by bilateral electric lesions of the nucleus Basalis of Meynert in rat. Neurochem Res. 2014;39(2):353-60. doi: 10.1007/s11064-013-1232-8. [PubMed: 24379110].
- Rabiei Z, Naderi S, Rafieian-Kopaei M. Study of antidepressant effects of grape seed oil in male mice using tail suspension and forced swim tests. Bangladesh J Pharmacol. 2017;12(4):397-402. doi: 10.3329/bjp.v12i4.33520.
- Rahnama S, Rabiei Z, Alibabaei Z, Mokhtari S, Rafieian-kopaei M, Deris F. Anti-amnesic activity of Citrus aurantium flowers extract against scopolamine-induced memory impairments in rats. Neurol Sci. 2015;36(4):553-60. doi: 10.1007/s10072-014-1991-2. [PubMed: 25367404].
- Nassiri-Asl M, Moghbelinejad S, Abbasi E, Yonesi F, Haghighi M-R, Lotfizadeh M. Effects of quercetin on oxidative stress and memory retrieval in kindled rats. Epilepsy Behav. 2013;28(2):151-5. doi: 10.1016/j.yebeh.2013.04.019. [PubMed: 23747498].
- Shin EJ, Jeong JH, Chung YH, Kim WK, Ko KH, Bach JH. Role of oxidative stress in epileptic seizures. Neurochem Int. 2011;59(2):122-37. doi: 10.1016/j.neuint.2011.03.025. [PubMed: 21672578].
- Helmstaedter C, Roeske S, Kaaden S, Elger CE, Schramm J. Hippocampal resection length and memory outcome in selective epilepsy surgery. J Neurol Neurosurg Psychiatry. 2011;82(12):1375-81. doi: 10.1136/jnnp.2010.240176. [PubMed: 21653207].
- Liu J, Yu H, Ning X. Effect of quercetin on chronic enhancement of spatial learning and memory of mice. Sci China C Life Sci. 2006;49(6):583-90. doi: 10.1007/s11427-006-2037-7. [PubMed: 17312997].
- Richetti S, Blank M, Capiotti K, Piato A, Bogo M, Vianna M. Quercetin and rutin prevent scopolamine-induced memory impairment in zebrafish. Behav Brain Res. 2011;217(1):10-5. doi: 10.1016/j.bbr.2010.09.027. [PubMed: 20888863].
- Mendez MF, Cummings JL, Benson DF. Depression in epilepsy: significance and phenomenology. Arch Neurol. 1986;43(8):766-70. doi: 10.1001/archneur.1986.00520080014012. [PubMed: 3729756].
- Bhutada P, Mundhada Y, Bansod K, Ubgade A, Quazi M, Umathe S. Reversal by quercetin of corticotrophin releasing factor induced anxiety-and depression-like effect in mice. Prog Neuropsychopharmacol Biol Psychiatry. 2010;34(6):955-60. doi: 10.1016/j.pnpbp.2010.04.025. [PubMed: 20447436].
- Anjaneyulu M, Chopra K, Kaur I. Antidepressant activity of quercetin, a bioflavonoid, in streptozotocin-induced diabetic mice. J Med Food. 2003;6(4):391-5. doi: 10.1089/109662003772519976. [PubMed: 14977450].
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