Document Type : Research articles
Authors
1 Department of Genetics, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran
2 Stem Cell Technology Research Center, Tehran, Iran
3 Department of Biochemistry, Medical School, Cellular and Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
Abstract
Background: Mesenchymal stromal cells (MSCs) have high differentiation potential into different cell lines like osteoblasts. Os- teogenic differentiation can be regulated through various and complex mechanisms, such as changing the microRNAs expression level. Although platelet-rich plasma (PRP) has been used in MSCs’ osteogenic differentiation process, the molecular mechanisms underlying the effect of PRP induction of osteogenesis by microRNAs is not well understood. Objectives: We evaluated the effect of PRP on the expression of miR-29a, miR-29b, and miR-155 in the PRP-based osteogenic differen- tiation of human MSCs. Methods: This experimental study was conducted on healthy individuals referred to Taleghani Hospital in Ahvaz, Iran, for ab- dominoplasty from September 2017 to April 2018. Stromal cells were isolated from human adipose tissue and differentiated into osteoblasts. Effect of 10% PRP on osteoblasts differentiation was monitored by the measurement of alkaline phosphatase activity and calcium deposition. We also evaluated gene expression of the Runx2 and the OPN along with the expression of miRNAs. All tests
were performed in triplicate. Results: Treatment of MSCs with 10% PRP resulted in induction of osteogenic differentiation by a significant upregulation of the miR-29a/b (miR-29a: 5.27 (0.77), P < 0.01 (day 3) and 3.76 (0.124), P < 0.01 (day 14); miR-29b: 3.11 (0.35), P < 0.001 (day 3) and 4.25 (0.304), P < 0.01 (day 14)) and a significant downregulation in the miR-155 expression (0.62 (0.006), P < 0.001 (day 3) and 0.55 (0.114), P < 0.05 (day 14)). Conclusions: The remarkable rise in the expression of important osteoblast genes, alkaline phosphatase activity, and calcium depo- sition verified accelerated differentiation. The present study showed that microRNAs such as miR-29a/b and miR-155 play an active
role in the process of bone differentiation during PRP treatment, which in turn, affects mesenchymal cells signaling pathways.
Keywords
', './data/ircmj/coversheet/cover_en.jpg'))