Document Type : Research articles

Authors

• Maryam Moradinasab ¹
• Afshin Ostovar ¹
• Iraj Nabipour ²
• Seyed Sajjad Eghbali ³
• Katayoun Vahdat ¹
• Abbas Ghaderi ⁴
• Mohamad Reza Farzaneh ³
• Mohammad Reza Ravanbod ⁵

¹ The Persian Gulf Tropical Medicine Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, IR Iran

² The Persian Gulf Marine Biotechnology Research Center, the Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, IR Iran

³ Department of Pathology, Bushehr University of Medical Sciences, Bushehr, IR Iran

⁴ Shiraz Institute for Cancer Research, Shiraz University of Medical Sciences, Shiraz, IR Iran

⁵ Department of Oncology and Hematology, Bushehr University of Medical Sciences, Bushehr, IR Iran

Abstract

Background: TP53 tumor suppressor gene participates in several pathways involving in carcinogenesis such as cell cycle control, DNA repair, and apoptosis. A common TP53 SNP (guanine/cytosine nucleotide substitution at codon 72), rs1042522, affects the function of p53 protein and may influence tumor behavior in response to environmental carcinogens.
Objectives: This study investigates the association between TP53 codon 72 polymorphisms, tobacco smoking, and breast cancer risk in southern Iranian women from Bushehr.
Methods: A case-control study was conducted on 144 cases with histologically confirmed invasive breast carcinoma and 162 randomly selected healthy controls with no previous cancer history in their family. TP53 codon 72 genotype was determined by using restriction fragment length polymorphism- polymerase chain reaction (RFLP-PCR) technique.
Results: Analysis revealed that smoking frequency was significantly higher in cases compared to controls (OR = 2.31, 95%CI = 1.33-3.99, P = 0.003) and the association between smoking and breast cancer was only significant for individuals with Arg/Pro genotype (OR = 3.23, 95% CI = 1.47 - 7.06, P = 0.003). On the other hand, there was no statistically considerable difference in the allele and genotype distribution between cases and controls.
Conclusions: These results should be confirmed in larger studies, but suggest that TP53 Arg/Pro genotypemodifies the risk of breast cancer in tobacco smokers and causes significantly more susceptibility to breast cancer due to smoking. 

Keywords

• P53
• Breast Neoplasms
• Polymorphism
• Smoking